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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8464 | 5' | -58.5 | NC_002167.1 | + | 18254 | 0.71 | 0.225546 |
Target: 5'- aGCCUGa-GCguuaGUGGUGAUGGCGGAgGu -3' miRNA: 3'- -UGGACgaCGg---CACCACUGCCGCUUgC- -5' |
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8464 | 5' | -58.5 | NC_002167.1 | + | 16836 | 0.72 | 0.185652 |
Target: 5'- gGCCUGUggcgucucacuguaUGCCGUGGUGACuGUGAcUGa -3' miRNA: 3'- -UGGACG--------------ACGGCACCACUGcCGCUuGC- -5' |
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8464 | 5' | -58.5 | NC_002167.1 | + | 18429 | 1.09 | 0.000339 |
Target: 5'- uACCUGCUGCCGUGGUGACGGCGAACGg -3' miRNA: 3'- -UGGACGACGGCACCACUGCCGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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