Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8466 | 5' | -51.5 | NC_002167.1 | + | 39471 | 0.66 | 0.841052 |
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Target: 5'- -aCCGacauUgACCGUGUCAggacauGUGUGGUCCGa -3' miRNA: 3'- acGGC----AgUGGCGCAGU------UACAUUAGGU- -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 14949 | 0.67 | 0.781761 |
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Target: 5'- aGCCGUCGCCacUGUCGAgucugGUGAUgCu -3' miRNA: 3'- aCGGCAGUGGc-GCAGUUa----CAUUAgGu -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 14039 | 0.68 | 0.716287 |
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Target: 5'- -cCCGUCACCG-G-CGAaGUGAUCCGg -3' miRNA: 3'- acGGCAGUGGCgCaGUUaCAUUAGGU- -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 30621 | 0.69 | 0.67061 |
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Target: 5'- cUGCCGUCcagcCCGUGUCGuUGUc-UCCGa -3' miRNA: 3'- -ACGGCAGu---GGCGCAGUuACAuuAGGU- -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 6072 | 0.73 | 0.414939 |
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Target: 5'- aGCCGUCGCCGCGgggaAAUuucuuccGgcGUCCAu -3' miRNA: 3'- aCGGCAGUGGCGCag--UUA-------CauUAGGU- -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 31175 | 0.75 | 0.308113 |
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Target: 5'- cGCCuGUCAagCGUGUCAGUGUAGUCa- -3' miRNA: 3'- aCGG-CAGUg-GCGCAGUUACAUUAGgu -5' |
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| 8466 | 5' | -51.5 | NC_002167.1 | + | 20777 | 0.96 | 0.012938 |
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Target: 5'- uUGCCG-UACCGCGUCAAUGUAAUCCAg -3' miRNA: 3'- -ACGGCaGUGGCGCAGUUACAUUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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