Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8471 | 3' | -48.4 | NC_002167.1 | + | 2242 | 0.66 | 0.916671 |
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Target: 5'- gUGGcgCUGCgugaGCCUGAUUuCAACGCu -3' miRNA: 3'- -ACUuaGACGa---UGGGUUAGuGUUGCGu -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 9799 | 0.67 | 0.909059 |
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Target: 5'- gGAAUCUGCaAUCCAAUaugcggaCACAAUcgaGCAg -3' miRNA: 3'- aCUUAGACGaUGGGUUA-------GUGUUG---CGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 7777 | 0.67 | 0.900309 |
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Target: 5'- --cGUCUGCUcaaacaggcgcuugACCUGAUCaACAAUGCGg -3' miRNA: 3'- acuUAGACGA--------------UGGGUUAG-UGUUGCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 17912 | 0.67 | 0.894993 |
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Target: 5'- aGAAcUCUGCUAUCgCAAUUAUucagggcaaccuGGCGCAg -3' miRNA: 3'- aCUU-AGACGAUGG-GUUAGUG------------UUGCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 35002 | 0.67 | 0.878963 |
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Target: 5'- aGAA-CUGCuUAUCCAGaCACGAgGCAa -3' miRNA: 3'- aCUUaGACG-AUGGGUUaGUGUUgCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 17412 | 0.68 | 0.843361 |
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Target: 5'- cGAugccaugCUGCUGgCCGAUgugccuuaccCGCAGCGCAa -3' miRNA: 3'- aCUua-----GACGAUgGGUUA----------GUGUUGCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 12184 | 0.7 | 0.782349 |
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Target: 5'- aUGAGUCcaaGCUACcggcgaaaCCAAUCugGugGCAg -3' miRNA: 3'- -ACUUAGa--CGAUG--------GGUUAGugUugCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 10433 | 0.72 | 0.631629 |
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Target: 5'- uUGAGUCUGCUAggCAAUUACAA-GCAa -3' miRNA: 3'- -ACUUAGACGAUggGUUAGUGUUgCGU- -5' |
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| 8471 | 3' | -48.4 | NC_002167.1 | + | 9444 | 1.1 | 0.002579 |
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Target: 5'- cUGAAUCUGCUACCCAAUCACAACGCAa -3' miRNA: 3'- -ACUUAGACGAUGGGUUAGUGUUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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