Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8474 | 5' | -58.6 | NC_002167.1 | + | 38633 | 0.66 | 0.434386 |
Target: 5'- aGCCaaACUACUGGUGGGCUugauaaugaaaccgGAGUUa-- -3' miRNA: 3'- -CGGg-UGGUGACCGCCCGA--------------CUCGAaug -5' |
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8474 | 5' | -58.6 | NC_002167.1 | + | 6173 | 0.68 | 0.313534 |
Target: 5'- cGCCUuuUACUGGCGauucGUUGAGCUUGg -3' miRNA: 3'- -CGGGugGUGACCGCc---CGACUCGAAUg -5' |
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8474 | 5' | -58.6 | NC_002167.1 | + | 6743 | 0.73 | 0.136716 |
Target: 5'- aCCCGCCAuCUGGCGGGUucguUGGGUg--- -3' miRNA: 3'- cGGGUGGU-GACCGCCCG----ACUCGaaug -5' |
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8474 | 5' | -58.6 | NC_002167.1 | + | 15075 | 0.75 | 0.097138 |
Target: 5'- aGCCCGCCAgUGGUGGGCacuGGCgcACu -3' miRNA: 3'- -CGGGUGGUgACCGCCCGac-UCGaaUG- -5' |
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8474 | 5' | -58.6 | NC_002167.1 | + | 15018 | 1.11 | 0.000171 |
Target: 5'- uGCCCACCACUGGCGGGCUGAGCUUACa -3' miRNA: 3'- -CGGGUGGUGACCGCCCGACUCGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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