Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8475 | 5' | -50.3 | NC_002167.1 | + | 20785 | 1.16 | 0.000692 |
Target: 5'- aGGCGUAGGCCAUUCAAUAUCUGGCGCa -3' miRNA: 3'- -CCGCAUCCGGUAAGUUAUAGACCGCG- -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 27120 | 0.68 | 0.776959 |
Target: 5'- gGGCGauGGCCGUuggcuugacuUUAGUuacugugauucuGUCUGGCGUg -3' miRNA: 3'- -CCGCauCCGGUA----------AGUUA------------UAGACCGCG- -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 20821 | 0.68 | 0.787495 |
Target: 5'- cGGCaGUucAGGCCAgaUGAcAUCcGGCGCg -3' miRNA: 3'- -CCG-CA--UCCGGUaaGUUaUAGaCCGCG- -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 32833 | 0.68 | 0.797848 |
Target: 5'- cGGCGUuuGCCAgcagUCcuugucGUGUgCUGGCGUa -3' miRNA: 3'- -CCGCAucCGGUa---AGu-----UAUA-GACCGCG- -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 15550 | 0.67 | 0.817957 |
Target: 5'- aGCGaGGGCCAUUUAAgacgCaGGCGa -3' miRNA: 3'- cCGCaUCCGGUAAGUUaua-GaCCGCg -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 38823 | 0.66 | 0.855449 |
Target: 5'- uGCagAGGCCAUUCAAUGagUGGUc- -3' miRNA: 3'- cCGcaUCCGGUAAGUUAUagACCGcg -5' |
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8475 | 5' | -50.3 | NC_002167.1 | + | 36536 | 0.66 | 0.880847 |
Target: 5'- -aCGUaAGGCguUccUCGAUAUgCUGGCGUg -3' miRNA: 3'- ccGCA-UCCGguA--AGUUAUA-GACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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