Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8477 | 3' | -53.1 | NC_002167.1 | + | 20746 | 0.66 | 0.776844 |
Target: 5'- cCAGCACCgcgCCGG-AUGucaucuggccuGAAcUGCCGGa -3' miRNA: 3'- -GUCGUGGa--GGCUaUGC-----------CUUaACGGCC- -5' |
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8477 | 3' | -53.1 | NC_002167.1 | + | 13345 | 0.7 | 0.489161 |
Target: 5'- aGGUGCaggCCGAUAagUGGAAUcaUGCCGGg -3' miRNA: 3'- gUCGUGga-GGCUAU--GCCUUA--ACGGCC- -5' |
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8477 | 3' | -53.1 | NC_002167.1 | + | 23779 | 0.71 | 0.465072 |
Target: 5'- cCAGCGCCUCUGuauGUGCGGAugacccgcuccgcuGUU-CCGGc -3' miRNA: 3'- -GUCGUGGAGGC---UAUGCCU--------------UAAcGGCC- -5' |
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8477 | 3' | -53.1 | NC_002167.1 | + | 23675 | 1.11 | 0.000848 |
Target: 5'- cCAGCACCUCCGAUACGGAAUUGCCGGa -3' miRNA: 3'- -GUCGUGGAGGCUAUGCCUUAACGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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