Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 848 | 3' | -60.8 | NC_000898.1 | + | 10088 | 0.66 | 0.73799 |
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Target: 5'- cGGcAGG-GUGUAUGCGGCCAguCUCGaCGg -3' miRNA: 3'- uCC-UCCaCGCAUGUGCCGGU--GGGC-GC- -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 10416 | 0.66 | 0.717152 |
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Target: 5'- cGGAGGacGCGUucggucucuggaaGCACGGCUugcgucgcuucgaGCCCGUc -3' miRNA: 3'- uCCUCCa-CGCA-------------UGUGCCGG-------------UGGGCGc -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 3335 | 0.67 | 0.674585 |
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Target: 5'- cGGAGGgGCGUGgAgGGUCcggagcggcgacggaGCCCGCu -3' miRNA: 3'- uCCUCCaCGCAUgUgCCGG---------------UGGGCGc -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 156502 | 0.67 | 0.674585 |
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Target: 5'- cGGAGGgGCGUGgAgGGUCcggagcggcgacggaGCCCGCu -3' miRNA: 3'- uCCUCCaCGCAUgUgCCGG---------------UGGGCGc -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 11597 | 0.67 | 0.641231 |
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Target: 5'- --cAGGUGCGacgagGCACGGCgCagGCUCGCGg -3' miRNA: 3'- uccUCCACGCa----UGUGCCG-G--UGGGCGC- -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 5468 | 0.72 | 0.38417 |
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Target: 5'- uGGGucucacGUGCGUugGCGGCCAacCCCaGCGg -3' miRNA: 3'- -UCCuc----CACGCAugUGCCGGU--GGG-CGC- -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 158636 | 0.72 | 0.38417 |
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Target: 5'- uGGGucucacGUGCGUugGCGGCCAacCCCaGCGg -3' miRNA: 3'- -UCCuc----CACGCAugUGCCGGU--GGG-CGC- -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 1647 | 1.08 | 0.001501 |
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Target: 5'- cAGGAGGUGCGUACACGGCCACCCGCGu -3' miRNA: 3'- -UCCUCCACGCAUGUGCCGGUGGGCGC- -5' |
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| 848 | 3' | -60.8 | NC_000898.1 | + | 154815 | 1.08 | 0.001501 |
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Target: 5'- cAGGAGGUGCGUACACGGCCACCCGCGu -3' miRNA: 3'- -UCCUCCACGCAUGUGCCGGUGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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