Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8480 | 3' | -52.8 | NC_002167.1 | + | 10551 | 0.66 | 0.751408 |
Target: 5'- gAUCGCuCUGCAAGG-GCAGaauaccgaGCuGGCAa -3' miRNA: 3'- gUAGCG-GAUGUUUCuCGUCg-------CG-CUGU- -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 10920 | 0.67 | 0.695491 |
Target: 5'- --gCGCUaccuCGAAGAGCGccuGCGCGACc -3' miRNA: 3'- guaGCGGau--GUUUCUCGU---CGCGCUGu -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 15863 | 0.67 | 0.695491 |
Target: 5'- aGUCGaauucgACGuAGAGCAGCGCGGu- -3' miRNA: 3'- gUAGCgga---UGUuUCUCGUCGCGCUgu -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 5662 | 0.68 | 0.580079 |
Target: 5'- gAUCaGCg-GCAGAGAGCAGCuuucagGCGGCGc -3' miRNA: 3'- gUAG-CGgaUGUUUCUCGUCG------CGCUGU- -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 17204 | 0.69 | 0.523466 |
Target: 5'- aCGUCGaaugACGugguGGAGguGCGCGGCAc -3' miRNA: 3'- -GUAGCgga-UGUu---UCUCguCGCGCUGU- -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 38387 | 0.71 | 0.448166 |
Target: 5'- --gCGCCUcCAGAGcGgGGCGCGACu -3' miRNA: 3'- guaGCGGAuGUUUCuCgUCGCGCUGu -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 17084 | 0.71 | 0.417769 |
Target: 5'- cUAUCGCCUAUGAAGcGCaauGGCGCcGCAa -3' miRNA: 3'- -GUAGCGGAUGUUUCuCG---UCGCGcUGU- -5' |
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8480 | 3' | -52.8 | NC_002167.1 | + | 37076 | 1.09 | 0.001023 |
Target: 5'- cCAUCGCCUACAAAGAGCAGCGCGACAa -3' miRNA: 3'- -GUAGCGGAUGUUUCUCGUCGCGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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