Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8480 | 5' | -51.2 | NC_002167.1 | + | 17912 | 0.66 | 0.835708 |
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Target: 5'- gCUGUCGauUCGCUGGCccaGUGCGGuaucAGCa -3' miRNA: 3'- aGGUAGU--AGCGACUG---UACGUCu---UCGc -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 5401 | 0.66 | 0.833818 |
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Target: 5'- uUCCAugcugguUCAUCGCaccagcGCAUGCAGuagucaaAAGCGg -3' miRNA: 3'- -AGGU-------AGUAGCGac----UGUACGUC-------UUCGC- -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 16389 | 0.66 | 0.796199 |
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Target: 5'- aCCAg---CGCUGugGUGUAGcGGCu -3' miRNA: 3'- aGGUaguaGCGACugUACGUCuUCGc -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 11027 | 0.67 | 0.753607 |
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Target: 5'- gUCGUCA-CGCUGcGCAU-CAGAAGCu -3' miRNA: 3'- aGGUAGUaGCGAC-UGUAcGUCUUCGc -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 3009 | 0.68 | 0.685808 |
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Target: 5'- cUCGaCGUCGCcGACAaGguGAGGCGg -3' miRNA: 3'- aGGUaGUAGCGaCUGUaCguCUUCGC- -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 6439 | 0.71 | 0.557954 |
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Target: 5'- uUCCAUCGUcgaugauguuucCGCUGACGUGCuGu-GUGg -3' miRNA: 3'- -AGGUAGUA------------GCGACUGUACGuCuuCGC- -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 32490 | 0.72 | 0.490684 |
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Target: 5'- -gCAUgGUCGCUGGCuggAUGCAGaAAGCu -3' miRNA: 3'- agGUAgUAGCGACUG---UACGUC-UUCGc -5' |
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| 8480 | 5' | -51.2 | NC_002167.1 | + | 37110 | 1.1 | 0.001377 |
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Target: 5'- aUCCAUCAUCGCUGACAUGCAGAAGCGu -3' miRNA: 3'- -AGGUAGUAGCGACUGUACGUCUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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