Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8481 | 3' | -52.9 | NC_002169.1 | + | 86333 | 0.66 | 0.965952 |
Target: 5'- uUGGUAuuGUCGUcGUCGUCGUCgucgggauuaaaCUGUUGAg -3' miRNA: 3'- -ACCAU--CAGCA-UAGCAGCAG------------GGCGAUU- -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 74732 | 0.66 | 0.958884 |
Target: 5'- ----cGUCGUcGUCGUCGUCuCCGCc-- -3' miRNA: 3'- accauCAGCA-UAGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 117742 | 0.66 | 0.954997 |
Target: 5'- cGGUuuuugaGGUCG--UCGUCGUCgCCGCc-- -3' miRNA: 3'- aCCA------UCAGCauAGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 51431 | 0.67 | 0.950869 |
Target: 5'- gGGUAGggugcCGUugaCGUCGUCgCCGCa-- -3' miRNA: 3'- aCCAUCa----GCAua-GCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 49318 | 0.67 | 0.950869 |
Target: 5'- cGGUAucggcgccaucGUCGUcGUCGUCGUCgUCGCg-- -3' miRNA: 3'- aCCAU-----------CAGCA-UAGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 86748 | 0.67 | 0.946495 |
Target: 5'- aUGaGUcGUCGUcGUCGUCGUCgUCGUUGAa -3' miRNA: 3'- -AC-CAuCAGCA-UAGCAGCAG-GGCGAUU- -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 108894 | 0.68 | 0.926489 |
Target: 5'- -cGUuGUCGUcGUCGUCGUCgCCGCc-- -3' miRNA: 3'- acCAuCAGCA-UAGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 3199 | 0.69 | 0.888891 |
Target: 5'- aUGGUGGUUGUGaugauUCGUCGUCgUCGUc-- -3' miRNA: 3'- -ACCAUCAGCAU-----AGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 120186 | 0.7 | 0.850941 |
Target: 5'- -cGUAGUCGUcgUGUCGUUUgCGCUAAc -3' miRNA: 3'- acCAUCAGCAuaGCAGCAGG-GCGAUU- -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 99534 | 0.72 | 0.760303 |
Target: 5'- gUGGUGGUaGUGUCGUCGUCgUCGUc-- -3' miRNA: 3'- -ACCAUCAgCAUAGCAGCAG-GGCGauu -5' |
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8481 | 3' | -52.9 | NC_002169.1 | + | 2990 | 1.06 | 0.007023 |
Target: 5'- aUGGUAGUCGUAUCGUCGUCCCGCUAAu -3' miRNA: 3'- -ACCAUCAGCAUAGCAGCAGGGCGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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