Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8483 | 5' | -57.9 | NC_002169.1 | + | 77651 | 0.66 | 0.831501 |
Target: 5'- cGCAcUCGACGccguuaaacaaaugaUUGGC-GGGGCAuucuUCGAc -3' miRNA: 3'- uCGU-AGCUGC---------------AGCCGuCCCCGU----AGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 55327 | 0.66 | 0.802332 |
Target: 5'- uGGCGgugacgaCGACGacagCGGCGGcGGCAUCGc -3' miRNA: 3'- -UCGUa------GCUGCa---GCCGUCcCCGUAGCu -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 25343 | 0.66 | 0.801446 |
Target: 5'- uGCAUCacuuguaGGCGagCGGUccGGGCGUCGAc -3' miRNA: 3'- uCGUAG-------CUGCa-GCCGucCCCGUAGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 133654 | 0.67 | 0.784333 |
Target: 5'- -cCAUCGGCGUCGGCAuacaGGGUuuggcCGAc -3' miRNA: 3'- ucGUAGCUGCAGCCGUc---CCCGua---GCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 96909 | 0.67 | 0.775126 |
Target: 5'- cAGCGUCGucgacacCGUCGGCA---GCGUCGAc -3' miRNA: 3'- -UCGUAGCu------GCAGCCGUcccCGUAGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 30490 | 0.67 | 0.765794 |
Target: 5'- -cCAUCGGCGgCGGCAacgugagcGGcGGCGUCGu -3' miRNA: 3'- ucGUAGCUGCaGCCGU--------CC-CCGUAGCu -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 130781 | 0.67 | 0.746787 |
Target: 5'- cGC-UCGaaGCGUgGGCAGGacgcuguuGGCGUCGGu -3' miRNA: 3'- uCGuAGC--UGCAgCCGUCC--------CCGUAGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 6390 | 0.68 | 0.707656 |
Target: 5'- aGGC-UCGGCGgcggCGGCGGcGGCggCGAc -3' miRNA: 3'- -UCGuAGCUGCa---GCCGUCcCCGuaGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 49545 | 0.69 | 0.616756 |
Target: 5'- cGGCGUCGcCGccaaUGGCGGGGGCAa--- -3' miRNA: 3'- -UCGUAGCuGCa---GCCGUCCCCGUagcu -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 127949 | 0.72 | 0.469372 |
Target: 5'- cGGCggCGGCGgCGGCGGcGGCGUCGu -3' miRNA: 3'- -UCGuaGCUGCaGCCGUCcCCGUAGCu -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 85752 | 0.72 | 0.450985 |
Target: 5'- uGGCAUCGuCGUCGaaaAGGGGCGcaaauUCGAc -3' miRNA: 3'- -UCGUAGCuGCAGCcg-UCCCCGU-----AGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 85494 | 0.73 | 0.398322 |
Target: 5'- gAGCAUCGGCGgu-GCGGuGGGCAUCu- -3' miRNA: 3'- -UCGUAGCUGCagcCGUC-CCCGUAGcu -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 6945 | 0.75 | 0.319819 |
Target: 5'- cGGCAUCGAUGUCGaCAucGGCAUCGAc -3' miRNA: 3'- -UCGUAGCUGCAGCcGUccCCGUAGCU- -5' |
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8483 | 5' | -57.9 | NC_002169.1 | + | 6909 | 1.08 | 0.001967 |
Target: 5'- aAGCAUCGACGUCGGCAGGGGCAUCGAc -3' miRNA: 3'- -UCGUAGCUGCAGCCGUCCCCGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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