miRNA: 3'- aaaUCAUGUacUAgAAACGAAAGUAG- -5'
miRNA: 3'- aaaucAUGuacUAGAAACGAAAGUAG- -5'
miRNA: 3'- aaaUCA---UGUacUAGAAACGAAAGUAG- -5'
miRNA: 3'- aaauCAUGUACU--------AGAAACGaaAGUAG- -5'
miRNA: 3'- aaaucaUGUacUAGAAACGAAAGUAG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8485 | 3' | -40.7 | NC_002169.1 | + | 105322 | 0.71 | 0.999993 |
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Target: 5'- --aAGUACAcaAUgUUUGCUUUCGUCc -3' miRNA: 3'- aaaUCAUGUacUAgAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 53091 | 0.7 | 0.999995 |
|
Target: 5'- ----aUACucaAUCUUUGCUUUCAUCg -3' miRNA: 3'- aaaucAUGuacUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 42453 | 0.7 | 0.999999 |
|
Target: 5'- --aAGUcaaACAcaAUCUUUGCUUUCGUCc -3' miRNA: 3'- aaaUCA---UGUacUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 10326 | 0.69 | 1 |
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Target: 5'- ---cGUACAUGAacaaagcaUCUUUGUcaUCGUCa -3' miRNA: 3'- aaauCAUGUACU--------AGAAACGaaAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 42376 | 0.68 | 1 |
|
Target: 5'- -----cACA--AUCUUUGCUUUCGUCc -3' miRNA: 3'- aaaucaUGUacUAGAAACGAAAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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