miRNA: 3'- aaaucAUGUaCUAGAAACGAAAGUAG- -5'
miRNA: 3'- -AAAUCAUGuaCUAGAAACGAAAGUAG- -5'
miRNA: 3'- aaaucAUGUACUAGAAACGAAAGUAG- -5'
miRNA: 3'- -AAAUCAUGUacUAGAAACGAAAGUAG- -5'
miRNA: 3'- aaaucaUGUacUAGAAACGAAAGUAG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8485 | 3' | -40.7 | NC_002169.1 | + | 120230 | 0.83 | 0.913463 |
|
Target: 5'- ----aUACAcGAUCUUUGCUUUCGUCg -3' miRNA: 3'- aaaucAUGUaCUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 42065 | 0.9 | 0.606345 |
|
Target: 5'- aUUUAGUACucGAUCUUUGCUUUCGUCc -3' miRNA: 3'- -AAAUCAUGuaCUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 53233 | 0.91 | 0.559444 |
|
Target: 5'- ----aUACAUGAUCUUUGCUUUCGUCc -3' miRNA: 3'- aaaucAUGUACUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 52978 | 0.91 | 0.536357 |
|
Target: 5'- uUUUAGUACAccAUCUUUGCUUUCAUCg -3' miRNA: 3'- -AAAUCAUGUacUAGAAACGAAAGUAG- -5' |
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| 8485 | 3' | -40.7 | NC_002169.1 | + | 42376 | 0.68 | 1 |
|
Target: 5'- -----cACA--AUCUUUGCUUUCGUCc -3' miRNA: 3'- aaaucaUGUacUAGAAACGAAAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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