Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8491 | 3' | -50.3 | NC_002169.1 | + | 106618 | 0.66 | 0.991732 |
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Target: 5'- -aGGCGGCG--ACagGGACgAUGUCg -3' miRNA: 3'- cgUCGCCGCauUGaaCCUGaUACAGa -5' |
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| 8491 | 3' | -50.3 | NC_002169.1 | + | 48936 | 0.67 | 0.987628 |
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Target: 5'- uGCGGCGGCGUA----GGGC-AUGUg- -3' miRNA: 3'- -CGUCGCCGCAUugaaCCUGaUACAga -5' |
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| 8491 | 3' | -50.3 | NC_002169.1 | + | 36218 | 0.68 | 0.972089 |
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Target: 5'- aCGGCGGCGc-ACaUGGACguuuUGUCg -3' miRNA: 3'- cGUCGCCGCauUGaACCUGau--ACAGa -5' |
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| 8491 | 3' | -50.3 | NC_002169.1 | + | 22496 | 0.69 | 0.962295 |
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Target: 5'- -aAGCgGGCGUAG--UGGACUAUcGUCa -3' miRNA: 3'- cgUCG-CCGCAUUgaACCUGAUA-CAGa -5' |
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| 8491 | 3' | -50.3 | NC_002169.1 | + | 79703 | 0.69 | 0.962295 |
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Target: 5'- aGCAuuuGCGGCGUcAAUUgGGAC-AUGUCg -3' miRNA: 3'- -CGU---CGCCGCA-UUGAaCCUGaUACAGa -5' |
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| 8491 | 3' | -50.3 | NC_002169.1 | + | 12712 | 1.1 | 0.007641 |
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Target: 5'- gGCAGCGGCGUAACUUGGACUAUGUCUu -3' miRNA: 3'- -CGUCGCCGCAUUGAACCUGAUACAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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