Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8492 | 3' | -58.5 | NC_002169.1 | + | 43497 | 0.67 | 0.728918 |
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Target: 5'- uUGCCcaagguGUACg-UGUCGAUGGGGGGCg -3' miRNA: 3'- cGCGGc-----CGUGgaACAGCUGCCCCUUG- -5' |
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| 8492 | 3' | -58.5 | NC_002169.1 | + | 77825 | 0.68 | 0.689583 |
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Target: 5'- uGC-CCGGCGuuUUaccGUCGACGGcGGGAUc -3' miRNA: 3'- -CGcGGCCGUggAA---CAGCUGCC-CCUUG- -5' |
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| 8492 | 3' | -58.5 | NC_002169.1 | + | 98705 | 0.68 | 0.65856 |
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Target: 5'- -aGCCGGgACCuUUGUCGauccgaaGCGGGGucuCa -3' miRNA: 3'- cgCGGCCgUGG-AACAGC-------UGCCCCuu-G- -5' |
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| 8492 | 3' | -58.5 | NC_002169.1 | + | 95274 | 0.71 | 0.529826 |
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Target: 5'- -aGUCGGCG--UUGUCGACGGGcuGAACg -3' miRNA: 3'- cgCGGCCGUggAACAGCUGCCC--CUUG- -5' |
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| 8492 | 3' | -58.5 | NC_002169.1 | + | 21553 | 0.72 | 0.472844 |
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Target: 5'- cGUGgCGGCcuACUUUGUCGAaUGGGGAGu -3' miRNA: 3'- -CGCgGCCG--UGGAACAGCU-GCCCCUUg -5' |
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| 8492 | 3' | -58.5 | NC_002169.1 | + | 21756 | 1.1 | 0.001274 |
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Target: 5'- gGCGCCGGCACCUUGUCGACGGGGAACu -3' miRNA: 3'- -CGCGGCCGUGGAACAGCUGCCCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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