Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8492 | 5' | -51 | NC_002169.1 | + | 82521 | 0.66 | 0.991454 |
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Target: 5'- aACACcgCCaccauaaCAUUCGACGAucu-GGCCg -3' miRNA: 3'- -UGUGa-GGg------GUAAGCUGUUucauCCGG- -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 51138 | 0.66 | 0.990208 |
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Target: 5'- aACGCggCgCCAaagUCGAUAGAGgcGGCg -3' miRNA: 3'- -UGUGa-GgGGUa--AGCUGUUUCauCCGg -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 36145 | 0.66 | 0.988823 |
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Target: 5'- cGCAUUCgCCcgUCu-CAAAGUuuGGCCg -3' miRNA: 3'- -UGUGAGgGGuaAGcuGUUUCAu-CCGG- -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 61062 | 0.66 | 0.988823 |
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Target: 5'- cAUGCUUUgCGaUCGACAAAGUugcucguGGCCu -3' miRNA: 3'- -UGUGAGGgGUaAGCUGUUUCAu------CCGG- -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 95266 | 0.66 | 0.987291 |
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Target: 5'- gACACUCCag--UCGGCGuuGUcgacGGGCUg -3' miRNA: 3'- -UGUGAGGgguaAGCUGUuuCA----UCCGG- -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 43867 | 0.67 | 0.981714 |
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Target: 5'- cACACUCCCa--UCGAU--GGUAGGg- -3' miRNA: 3'- -UGUGAGGGguaAGCUGuuUCAUCCgg -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 21591 | 0.77 | 0.587492 |
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Target: 5'- gACAgUUCCCcgUCGACAAGGUgccGGcGCCg -3' miRNA: 3'- -UGUgAGGGGuaAGCUGUUUCA---UC-CGG- -5' |
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| 8492 | 5' | -51 | NC_002169.1 | + | 21720 | 1.13 | 0.00425 |
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Target: 5'- uACACUCCCCAUUCGACAAAGUAGGCCg -3' miRNA: 3'- -UGUGAGGGGUAAGCUGUUUCAUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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