Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8493 | 3' | -56.2 | NC_002169.1 | + | 6945 | 0.66 | 0.877039 |
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Target: 5'- --cGGCAucgaUGUCGA-CAUCGGCAuCGAc -3' miRNA: 3'- uaaCCGU----ACGGCUgGUAGCCGU-GCUc -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 27612 | 0.66 | 0.854167 |
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Target: 5'- --cGGgAUGCCGGCUGaCGGCGCc-- -3' miRNA: 3'- uaaCCgUACGGCUGGUaGCCGUGcuc -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 122017 | 0.66 | 0.854167 |
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Target: 5'- --cGGCcaaaacGCuCGGCCGUUGGCgGCGAGc -3' miRNA: 3'- uaaCCGua----CG-GCUGGUAGCCG-UGCUC- -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 90239 | 0.66 | 0.837869 |
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Target: 5'- -gUGGUcUGaCGACUAUCGGCGCuGAc -3' miRNA: 3'- uaACCGuACgGCUGGUAGCCGUG-CUc -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 60056 | 0.66 | 0.837869 |
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Target: 5'- --cGGUgAUGCCGuuuucGCCAUCGGuCGCaGAGu -3' miRNA: 3'- uaaCCG-UACGGC-----UGGUAGCC-GUG-CUC- -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 64157 | 0.67 | 0.820797 |
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Target: 5'- ---cGCAUGCCaggucgGACCAuUCGGCcaauGCGAGa -3' miRNA: 3'- uaacCGUACGG------CUGGU-AGCCG----UGCUC- -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 28053 | 0.68 | 0.754948 |
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Target: 5'- --aGGCgaucgacgcgcucGUGCCGAUgGUCGGCAUGc- -3' miRNA: 3'- uaaCCG-------------UACGGCUGgUAGCCGUGCuc -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 128606 | 0.68 | 0.736226 |
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Target: 5'- --aGGCAUuCCGAUCGUCGGacgGCGAu -3' miRNA: 3'- uaaCCGUAcGGCUGGUAGCCg--UGCUc -5' |
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| 8493 | 3' | -56.2 | NC_002169.1 | + | 28228 | 1.06 | 0.003491 |
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Target: 5'- cAUUGGCAUGCCGACCAUCGGCACGAGc -3' miRNA: 3'- -UAACCGUACGGCUGGUAGCCGUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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