Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8493 | 5' | -58.4 | NC_002169.1 | + | 120912 | 0.66 | 0.79429 |
Target: 5'- cGUUUUGcugCCGUgGCUgCUGCucaucCUCCGCCu -3' miRNA: 3'- -CGGAGCa--GGCA-CGA-GACGu----GAGGCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 124416 | 0.68 | 0.709377 |
Target: 5'- -gCUCGUCg--GCUCUGCGCUagCGUCg -3' miRNA: 3'- cgGAGCAGgcaCGAGACGUGAg-GCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 46611 | 0.68 | 0.669603 |
Target: 5'- cGCCgguUCGUCgGUcgagguagcgacGCUcCUGCAUaCCGCCa -3' miRNA: 3'- -CGG---AGCAGgCA------------CGA-GACGUGaGGCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 79725 | 0.69 | 0.609211 |
Target: 5'- aGCCUCGUUCGU-CUauaGCGUUCUGCCg -3' miRNA: 3'- -CGGAGCAGGCAcGAga-CGUGAGGCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 88921 | 0.72 | 0.445527 |
Target: 5'- -gCUCGUCUGUGCcgaCUGUAUUUCGUCg -3' miRNA: 3'- cgGAGCAGGCACGa--GACGUGAGGCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 35472 | 0.76 | 0.295605 |
Target: 5'- cGCCUaacuuugacUGUaCCGUGCUCUGCGauaaucCCGCCg -3' miRNA: 3'- -CGGA---------GCA-GGCACGAGACGUga----GGCGG- -5' |
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8493 | 5' | -58.4 | NC_002169.1 | + | 28192 | 1.13 | 0.000942 |
Target: 5'- cGCCUCGUCCGUGCUCUGCACUCCGCCc -3' miRNA: 3'- -CGGAGCAGGCACGAGACGUGAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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