Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8495 | 5' | -59.8 | NC_002169.1 | + | 28579 | 0.66 | 0.779853 |
Target: 5'- -aCGCGCgCGcacaaaACGCCUAC-UGCGCa- -3' miRNA: 3'- ggGCGCGaGC------UGCGGAUGcACGCGcu -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 22311 | 0.66 | 0.76167 |
Target: 5'- gCCCGCGCUCacUGCCgaagucaggGCGUaauaucgguugGUGCGGg -3' miRNA: 3'- -GGGCGCGAGcuGCGGa--------UGCA-----------CGCGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 22118 | 0.66 | 0.759827 |
Target: 5'- -aCGCGCUCGGCGCCcgcaccaaccgauauUACGcccugacuucggcagUgaGCGCGGg -3' miRNA: 3'- ggGCGCGAGCUGCGG---------------AUGC---------------A--CGCGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 58974 | 0.66 | 0.743056 |
Target: 5'- aUCUGCGC--GACaGCCUGCGcGCGCu- -3' miRNA: 3'- -GGGCGCGagCUG-CGGAUGCaCGCGcu -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 56754 | 0.66 | 0.733609 |
Target: 5'- gCCCGCGUuuacgagaUCGGCGUCgAUGUGCuguuCGAc -3' miRNA: 3'- -GGGCGCG--------AGCUGCGGaUGCACGc---GCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 78032 | 0.67 | 0.695078 |
Target: 5'- gCCCGacgaaCGCUuCGACGCgaccucgaaUACGUGCGUGc -3' miRNA: 3'- -GGGC-----GCGA-GCUGCGg--------AUGCACGCGCu -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 5483 | 0.67 | 0.695078 |
Target: 5'- -gUGUGUUCGACGCCguuCGUGCaCGu -3' miRNA: 3'- ggGCGCGAGCUGCGGau-GCACGcGCu -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 22387 | 0.67 | 0.675484 |
Target: 5'- aUUGCGCUCGGCGUCgccaugUACG-GcCGCGGa -3' miRNA: 3'- gGGCGCGAGCUGCGG------AUGCaC-GCGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 124251 | 0.68 | 0.653779 |
Target: 5'- --aGCGCUCGACGCUagcgcagagccgACGaGCGUGAc -3' miRNA: 3'- gggCGCGAGCUGCGGa-----------UGCaCGCGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 57938 | 0.69 | 0.596384 |
Target: 5'- uUCGCGUUCGAaauguccgaCGCCgACGUGCaCGAc -3' miRNA: 3'- gGGCGCGAGCU---------GCGGaUGCACGcGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 48139 | 0.73 | 0.354986 |
Target: 5'- aCCGCcgUCGGCGCCguCGUGUGCGAc -3' miRNA: 3'- gGGCGcgAGCUGCGGauGCACGCGCU- -5' |
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8495 | 5' | -59.8 | NC_002169.1 | + | 35002 | 1.1 | 0.00114 |
Target: 5'- cCCCGCGCUCGACGCCUACGUGCGCGAa -3' miRNA: 3'- -GGGCGCGAGCUGCGGAUGCACGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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