Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8497 | 3' | -52.2 | NC_002169.1 | + | 96525 | 0.66 | 0.967811 |
Target: 5'- cGCGACGCGcUCUugccgcauuacaaGUAcgugucguuguguaaGAAGGGGGUg -3' miRNA: 3'- aCGCUGCGCaAGA-------------CGU---------------UUUCCCUCAa -5' |
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8497 | 3' | -52.2 | NC_002169.1 | + | 81751 | 0.7 | 0.833196 |
Target: 5'- cGuCGACGUGUucuuagauaUCUGCAGGAuGGGGGa- -3' miRNA: 3'- aC-GCUGCGCA---------AGACGUUUU-CCCUCaa -5' |
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8497 | 3' | -52.2 | NC_002169.1 | + | 19682 | 0.74 | 0.631507 |
Target: 5'- gUGCGGCGCGUguuggcggUUUGCGAGucuGGGAGc- -3' miRNA: 3'- -ACGCUGCGCA--------AGACGUUUu--CCCUCaa -5' |
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8497 | 3' | -52.2 | NC_002169.1 | + | 37135 | 1.05 | 0.008247 |
Target: 5'- aUGCGACGCGUUCUGCAAAAGGGAGUUu -3' miRNA: 3'- -ACGCUGCGCAAGACGUUUUCCCUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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