Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8498 | 3' | -57 | NC_002169.1 | + | 125337 | 0.67 | 0.779559 |
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Target: 5'- cCUCGCCGAuacUGCC-CCAACC--GCCGa -3' miRNA: 3'- -GAGUGGCU---GCGGaGGUUGGucUGGUa -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 45969 | 0.68 | 0.731379 |
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Target: 5'- --gACCGACGUCUCgGACUGGGCgAg -3' miRNA: 3'- gagUGGCUGCGGAGgUUGGUCUGgUa -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 22572 | 0.68 | 0.731379 |
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Target: 5'- gCUCA-CGACGCCgcUCCAuCCGcGGCCGUa -3' miRNA: 3'- -GAGUgGCUGCGG--AGGUuGGU-CUGGUA- -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 37963 | 0.69 | 0.670787 |
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Target: 5'- aUCACCGAgGcCCUUCAACUGGcCCGa -3' miRNA: 3'- gAGUGGCUgC-GGAGGUUGGUCuGGUa -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 86090 | 0.69 | 0.650238 |
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Target: 5'- aCUgGCCGGgGCCUCC-GCaCAGAUCGa -3' miRNA: 3'- -GAgUGGCUgCGGAGGuUG-GUCUGGUa -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 25831 | 0.71 | 0.557961 |
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Target: 5'- --aGCCGGCGCCgCCGACCGG-CUAa -3' miRNA: 3'- gagUGGCUGCGGaGGUUGGUCuGGUa -5' |
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| 8498 | 3' | -57 | NC_002169.1 | + | 39408 | 1.05 | 0.003266 |
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Target: 5'- aCUCACCGACGCCUCCAACCAGACCAUc -3' miRNA: 3'- -GAGUGGCUGCGGAGGUUGGUCUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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