Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8499 | 5' | -55.3 | NC_002169.1 | + | 98073 | 0.66 | 0.921138 |
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Target: 5'- cCAUGUUgAcgGGCAC-UCGGUGUCCa -3' miRNA: 3'- -GUGCAGgUugUCGUGaAGCCACGGGg -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 103062 | 0.66 | 0.896709 |
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Target: 5'- cCACGUCCAuucuauuGUugUuugUCGGUGCCa- -3' miRNA: 3'- -GUGCAGGUugu----CGugA---AGCCACGGgg -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 112231 | 0.68 | 0.811181 |
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Target: 5'- aACGUCguCGGCAGCAaUUCGG-GCCgCg -3' miRNA: 3'- gUGCAG--GUUGUCGUgAAGCCaCGGgG- -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 63298 | 0.69 | 0.774599 |
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Target: 5'- gGCGUCUAgagcuGCGGCGCUUgcgUGGccUGCCCg -3' miRNA: 3'- gUGCAGGU-----UGUCGUGAA---GCC--ACGGGg -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 60213 | 0.69 | 0.755492 |
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Target: 5'- aACGUCCA--GGgAaggUCGGUGCCCa -3' miRNA: 3'- gUGCAGGUugUCgUga-AGCCACGGGg -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 99300 | 0.71 | 0.684605 |
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Target: 5'- aGCGUaaauacaucaaagUCAACGGCGagUgGGUGCCCCg -3' miRNA: 3'- gUGCA-------------GGUUGUCGUgaAgCCACGGGG- -5' |
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| 8499 | 5' | -55.3 | NC_002169.1 | + | 41676 | 1.12 | 0.001864 |
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Target: 5'- gCACGUCCAACAGCACUUCGGUGCCCCa -3' miRNA: 3'- -GUGCAGGUUGUCGUGAAGCCACGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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