Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
850 | 3' | -58.3 | NC_000898.1 | + | 2802 | 0.66 | 0.825091 |
Target: 5'- -aGGGGACGGACAGaGcGGAgaggCG-CGGg -3' miRNA: 3'- gaCCCCUGUCUGUCcC-CCUa---GCuGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 155970 | 0.66 | 0.825091 |
Target: 5'- -aGGGGACGGACAGaGcGGAgaggCG-CGGg -3' miRNA: 3'- gaCCCCUGUCUGUCcC-CCUa---GCuGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 2754 | 0.67 | 0.808254 |
Target: 5'- -cGGGcGACGGGCAGGGcGGG-C-ACGGa -3' miRNA: 3'- gaCCC-CUGUCUGUCCC-CCUaGcUGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 155922 | 0.67 | 0.808254 |
Target: 5'- -cGGGcGACGGGCAGGGcGGG-C-ACGGa -3' miRNA: 3'- gaCCC-CUGUCUGUCCC-CCUaGcUGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 3655 | 0.67 | 0.790812 |
Target: 5'- -cGGGGcggauCGGGCGGGGGGcgCagaGCGGa -3' miRNA: 3'- gaCCCCu----GUCUGUCCCCCuaGc--UGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 156823 | 0.67 | 0.790812 |
Target: 5'- -cGGGGcggauCGGGCGGGGGGcgCagaGCGGa -3' miRNA: 3'- gaCCCCu----GUCUGUCCCCCuaGc--UGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 649 | 0.68 | 0.735502 |
Target: 5'- -cGGuGGCGGugGGGGGGAU-GugGGu -3' miRNA: 3'- gaCCcCUGUCugUCCCCCUAgCugCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 153817 | 0.68 | 0.735502 |
Target: 5'- -cGGuGGCGGugGGGGGGAU-GugGGu -3' miRNA: 3'- gaCCcCUGUCugUCCCCCUAgCugCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 37826 | 0.69 | 0.696813 |
Target: 5'- -aGGGGACcGAuuguccuuCAGGGGGcgCGGgGAc -3' miRNA: 3'- gaCCCCUGuCU--------GUCCCCCuaGCUgCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 3867 | 0.7 | 0.607534 |
Target: 5'- -gGGGGAUGGAgggaGGGGGGAcCGAgGGg -3' miRNA: 3'- gaCCCCUGUCUg---UCCCCCUaGCUgCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 157035 | 0.7 | 0.607534 |
Target: 5'- -gGGGGAUGGAgggaGGGGGGAcCGAgGGg -3' miRNA: 3'- gaCCCCUGUCUg---UCCCCCUaGCUgCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 4631 | 0.76 | 0.325129 |
Target: 5'- -aGGGGAUgaGGGCGGGGGGAagGGCa- -3' miRNA: 3'- gaCCCCUG--UCUGUCCCCCUagCUGcu -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 157799 | 0.76 | 0.325129 |
Target: 5'- -aGGGGAUgaGGGCGGGGGGAagGGCa- -3' miRNA: 3'- gaCCCCUG--UCUGUCCCCCUagCUGcu -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 2591 | 1.08 | 0.002353 |
Target: 5'- cCUGGGGACAGACAGGGGGAUCGACGAc -3' miRNA: 3'- -GACCCCUGUCUGUCCCCCUAGCUGCU- -5' |
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850 | 3' | -58.3 | NC_000898.1 | + | 155759 | 1.08 | 0.002353 |
Target: 5'- cCUGGGGACAGACAGGGGGAUCGACGAc -3' miRNA: 3'- -GACCCCUGUCUGUCCCCCUAGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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