Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
850 | 5' | -56.8 | NC_000898.1 | + | 3812 | 0.66 | 0.911478 |
Target: 5'- cGUUCGggggaCU-UCCCGCCGacGCGcCUGCg -3' miRNA: 3'- -CAAGCg----GAgAGGGCGGCa-UGCaGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 156980 | 0.66 | 0.911478 |
Target: 5'- cGUUCGggggaCU-UCCCGCCGacGCGcCUGCg -3' miRNA: 3'- -CAAGCg----GAgAGGGCGGCa-UGCaGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 7075 | 0.66 | 0.906093 |
Target: 5'- --aCGCCUC-CgCUGCCuaucggaggcgacgGCGUCUGCg -3' miRNA: 3'- caaGCGGAGaG-GGCGGca------------UGCAGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 160242 | 0.66 | 0.906093 |
Target: 5'- --aCGCCUC-CgCUGCCuaucggaggcgacgGCGUCUGCg -3' miRNA: 3'- caaGCGGAGaG-GGCGGca------------UGCAGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 38643 | 0.66 | 0.886145 |
Target: 5'- --gCGUUUCUCCUGCCGUuuAC-UCUGa -3' miRNA: 3'- caaGCGGAGAGGGCGGCA--UGcAGACg -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 111805 | 0.67 | 0.841727 |
Target: 5'- --aCGCCUUUCCCGggaUGUACcgagGUUUGCu -3' miRNA: 3'- caaGCGGAGAGGGCg--GCAUG----CAGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 2554 | 1.12 | 0.001847 |
Target: 5'- uGUUCGCCUCUCCCGCCGUACGUCUGCg -3' miRNA: 3'- -CAAGCGGAGAGGGCGGCAUGCAGACG- -5' |
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850 | 5' | -56.8 | NC_000898.1 | + | 155722 | 1.12 | 0.001847 |
Target: 5'- uGUUCGCCUCUCCCGCCGUACGUCUGCg -3' miRNA: 3'- -CAAGCGGAGAGGGCGGCAUGCAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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