Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8501 | 3' | -51.4 | NC_002169.1 | + | 116101 | 0.66 | 0.987665 |
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Target: 5'- -uCGUagUCGAAuaugucGCG-GCCGUCUCGCu- -3' miRNA: 3'- cuGCA--AGCUU------UGCaCGGCAGAGUGca -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 90960 | 0.66 | 0.987665 |
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Target: 5'- cGGCGUUCGAAAacUGCUGUCcauucgagUACGUu -3' miRNA: 3'- -CUGCAAGCUUUgcACGGCAGa-------GUGCA- -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 108054 | 0.66 | 0.987665 |
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Target: 5'- cGAgGUUCcGAACGggcGUCGUCUCGCc- -3' miRNA: 3'- -CUgCAAGcUUUGCa--CGGCAGAGUGca -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 108420 | 0.67 | 0.982276 |
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Target: 5'- cGACaaucaaCGGGACGUGCUGUucgCUCGCGa -3' miRNA: 3'- -CUGcaa---GCUUUGCACGGCA---GAGUGCa -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 65686 | 0.68 | 0.969619 |
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Target: 5'- cGACGaUUCGAgguaguagaagAGCGUGCCGUC-CAaauUGUa -3' miRNA: 3'- -CUGC-AAGCU-----------UUGCACGGCAGaGU---GCA- -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 57936 | 0.68 | 0.959473 |
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Target: 5'- cGCGUUCGAAAUGUccgacGCCGaCgugCACGa -3' miRNA: 3'- cUGCAAGCUUUGCA-----CGGCaGa--GUGCa -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 2957 | 0.69 | 0.932715 |
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Target: 5'- gGGCGaguaauagUCG-AGCGUGCCGUCgagaCACGa -3' miRNA: 3'- -CUGCa-------AGCuUUGCACGGCAGa---GUGCa -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 58233 | 0.7 | 0.927378 |
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Target: 5'- cGGCGUgggCGAAACGUGCgUGUUcaGCGUa -3' miRNA: 3'- -CUGCAa--GCUUUGCACG-GCAGagUGCA- -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 82927 | 0.79 | 0.493848 |
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Target: 5'- cACGUUCGGAGCGUGCgGUCauaGCGUc -3' miRNA: 3'- cUGCAAGCUUUGCACGgCAGag-UGCA- -5' |
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| 8501 | 3' | -51.4 | NC_002169.1 | + | 44119 | 1.07 | 0.009703 |
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Target: 5'- cGACGUUCGAAACGUGCCGUCUCACGUc -3' miRNA: 3'- -CUGCAAGCUUUGCACGGCAGAGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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