Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8501 | 5' | -53.5 | NC_002169.1 | + | 104209 | 0.66 | 0.94282 |
Target: 5'- aUGACUUgUGGGCACgCuCGGUCgUCGAa- -3' miRNA: 3'- -ACUGAA-ACUCGUG-GuGCCAG-GGCUaa -5' |
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8501 | 5' | -53.5 | NC_002169.1 | + | 125756 | 0.72 | 0.679538 |
Target: 5'- aUGGCUUUGuGCGCCACGGUaggaGGUa -3' miRNA: 3'- -ACUGAAACuCGUGGUGCCAggg-CUAa -5' |
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8501 | 5' | -53.5 | NC_002169.1 | + | 44085 | 1.06 | 0.005987 |
Target: 5'- cUGACUUUGAGCACCACGGUCCCGAUUg -3' miRNA: 3'- -ACUGAAACUCGUGGUGCCAGGGCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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