Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8508 | 3' | -53.4 | NC_002169.1 | + | 66286 | 0.66 | 0.951748 |
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Target: 5'- -aUUCGUGAgacGCgGCgCA-GGCCACg -3' miRNA: 3'- agAAGCACUaa-CGgCGgGUaUCGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 95555 | 0.67 | 0.909934 |
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Target: 5'- ---aCGUGGUcgUGCCGCCCAaaacgAGCauucaGCg -3' miRNA: 3'- agaaGCACUA--ACGGCGGGUa----UCGg----UG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 21921 | 0.67 | 0.90356 |
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Target: 5'- cUUUUGU-AUUGCgGCCCAcggaucguguaUAGCCACc -3' miRNA: 3'- aGAAGCAcUAACGgCGGGU-----------AUCGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 85228 | 0.68 | 0.896936 |
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Target: 5'- gCUUCGUuuagGAauaGCCGCUCGUuGGCCAUg -3' miRNA: 3'- aGAAGCA----CUaa-CGGCGGGUA-UCGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 54114 | 0.68 | 0.875595 |
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Target: 5'- --aUCGUG--UGCCGuCCCGcUGGCCAa -3' miRNA: 3'- agaAGCACuaACGGC-GGGU-AUCGGUg -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 49889 | 0.7 | 0.780859 |
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Target: 5'- gUCUUCGUuGUUGUCGgCCAU-GUCGCg -3' miRNA: 3'- -AGAAGCAcUAACGGCgGGUAuCGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 15826 | 0.7 | 0.771203 |
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Target: 5'- gUCgUCGUGAccGCCGCCgAcgcuGCCGCa -3' miRNA: 3'- -AGaAGCACUaaCGGCGGgUau--CGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 87498 | 0.71 | 0.761414 |
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Target: 5'- aUUUUCGUcuuuGAUgcacGCCGCCCAUcguuucaaguuGGCCAUa -3' miRNA: 3'- -AGAAGCA----CUAa---CGGCGGGUA-----------UCGGUG- -5' |
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| 8508 | 3' | -53.4 | NC_002169.1 | + | 86955 | 1.11 | 0.002835 |
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Target: 5'- gUCUUCGUGAUUGCCGCCCAUAGCCACg -3' miRNA: 3'- -AGAAGCACUAACGGCGGGUAUCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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