Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8510 | 5' | -42.8 | NC_002169.1 | + | 95145 | 0.66 | 1 |
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Target: 5'- gAUCUCcgUACA---GCAGACAuuCGAGAUCg -3' miRNA: 3'- -UAGAG--AUGUucuUGUUUGU--GUUCUAG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 9658 | 0.67 | 0.999999 |
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Target: 5'- ---cUUACAAGAGgAGAUACGAGGUg -3' miRNA: 3'- uagaGAUGUUCUUgUUUGUGUUCUAg -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 110051 | 0.67 | 0.999999 |
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Target: 5'- --aUCUACGAcGGCAAACgcuACAAGAUUg -3' miRNA: 3'- uagAGAUGUUcUUGUUUG---UGUUCUAG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 29543 | 0.67 | 0.999999 |
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Target: 5'- gGUUUCguggACGAGAACGAGgACGAGu-- -3' miRNA: 3'- -UAGAGa---UGUUCUUGUUUgUGUUCuag -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 129828 | 0.67 | 0.999998 |
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Target: 5'- uGUUUCgACu-GAACGAACGCGacgAGAUCg -3' miRNA: 3'- -UAGAGaUGuuCUUGUUUGUGU---UCUAG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 73825 | 0.68 | 0.999994 |
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Target: 5'- -gUUCUggcugacGCGAGAACAAuaGCGCGucGGAUCg -3' miRNA: 3'- uaGAGA-------UGUUCUUGUU--UGUGU--UCUAG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 128878 | 0.69 | 0.999977 |
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Target: 5'- uUUUC-GCGAGGGCAGACACAaaggccgccgguAGAUUg -3' miRNA: 3'- uAGAGaUGUUCUUGUUUGUGU------------UCUAG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 85318 | 0.72 | 0.999174 |
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Target: 5'- ---aCUACAGGGACAAuCGCGAGAcgcUCg -3' miRNA: 3'- uagaGAUGUUCUUGUUuGUGUUCU---AG- -5' |
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| 8510 | 5' | -42.8 | NC_002169.1 | + | 95217 | 1.1 | 0.037928 |
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Target: 5'- uAUCUCUACAAGAACAAACACAAGAUCg -3' miRNA: 3'- -UAGAGAUGUUCUUGUUUGUGUUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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