Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8511 | 3' | -60.9 | NC_002169.1 | + | 84524 | 0.66 | 0.646308 |
Target: 5'- cAAUaUCCGgGCGUGGcuaGCGACGUcaaguucGCCAa -3' miRNA: 3'- -UUGcAGGCgCGCACC---UGCUGCG-------CGGU- -5' |
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8511 | 3' | -60.9 | NC_002169.1 | + | 59960 | 0.68 | 0.528902 |
Target: 5'- -uCGaCCGa-CGUGGACGGCGuCGCCGu -3' miRNA: 3'- uuGCaGGCgcGCACCUGCUGC-GCGGU- -5' |
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8511 | 3' | -60.9 | NC_002169.1 | + | 77741 | 0.69 | 0.481728 |
Target: 5'- gAAUGUUCGaCGCGUcgucGGCGACGCGCg- -3' miRNA: 3'- -UUGCAGGC-GCGCAc---CUGCUGCGCGgu -5' |
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8511 | 3' | -60.9 | NC_002169.1 | + | 77911 | 0.79 | 0.127478 |
Target: 5'- cGCGUgCCGCGCGUcgccGACGACGCGUCGa -3' miRNA: 3'- uUGCA-GGCGCGCAc---CUGCUGCGCGGU- -5' |
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8511 | 3' | -60.9 | NC_002169.1 | + | 95500 | 1.05 | 0.001685 |
Target: 5'- uAACGUCCGCGCGUGGACGACGCGCCAu -3' miRNA: 3'- -UUGCAGGCGCGCACCUGCUGCGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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