Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8511 | 5' | -54.4 | NC_002169.1 | + | 37759 | 0.65 | 0.941023 |
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Target: 5'- uGUGGCaUCAGC-CCCAAGUCUaccgcagCGGcCg -3' miRNA: 3'- gCGUUG-AGUCGuGGGUUCAGA-------GCCuG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 64678 | 0.66 | 0.936646 |
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Target: 5'- aCGCGGCUUcugAGCAUCUguGAGUUUCuGACc -3' miRNA: 3'- -GCGUUGAG---UCGUGGG--UUCAGAGcCUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 128622 | 0.66 | 0.914746 |
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Target: 5'- aCGCAACUCacaauguaGGCAuUCCGA-UCgUCGGACg -3' miRNA: 3'- -GCGUUGAG--------UCGU-GGGUUcAG-AGCCUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 121073 | 0.68 | 0.850475 |
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Target: 5'- cCGCAACUCGucCACCCAAGUCaccgcucUCGucaccGACg -3' miRNA: 3'- -GCGUUGAGUc-GUGGGUUCAG-------AGC-----CUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 102598 | 0.7 | 0.771232 |
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Target: 5'- aGCAGCagCAGCAgCaCGGGUaaaUCGGGCa -3' miRNA: 3'- gCGUUGa-GUCGUgG-GUUCAg--AGCCUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 32979 | 0.7 | 0.741878 |
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Target: 5'- -aCAACaUCAGCGCCCAcgcGUCUCGacGACu -3' miRNA: 3'- gcGUUG-AGUCGUGGGUu--CAGAGC--CUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 29672 | 0.75 | 0.505268 |
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Target: 5'- aCGCGA-UCGGUugCCuuuuGUCUCGGGCg -3' miRNA: 3'- -GCGUUgAGUCGugGGuu--CAGAGCCUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 70118 | 0.75 | 0.466347 |
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Target: 5'- gGCgAGCUCGGCGCCCAAccgaauaUCGGGCa -3' miRNA: 3'- gCG-UUGAGUCGUGGGUUcag----AGCCUG- -5' |
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| 8511 | 5' | -54.4 | NC_002169.1 | + | 95463 | 1.1 | 0.002731 |
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Target: 5'- uCGCAACUCAGCACCCAAGUCUCGGACg -3' miRNA: 3'- -GCGUUGAGUCGUGGGUUCAGAGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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