Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8515 | 3' | -50.8 | NC_002169.1 | + | 4177 | 0.66 | 0.987863 |
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Target: 5'- gACCGGUuGCGGUGgugacggcggcGGAggcgaaggacUUGGUGAUGGu -3' miRNA: 3'- -UGGUCAuCGCUAC-----------UCU----------AACCGCUACU- -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 1549 | 0.67 | 0.977884 |
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Target: 5'- cAUUGGUGGCGGUGGc---GGCGGUGGc -3' miRNA: 3'- -UGGUCAUCGCUACUcuaaCCGCUACU- -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 1444 | 0.7 | 0.926179 |
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Target: 5'- cAUCGGUGGUGGaggagGAGGUggUGGUGGUGGu -3' miRNA: 3'- -UGGUCAUCGCUa----CUCUA--ACCGCUACU- -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 80340 | 0.7 | 0.926179 |
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Target: 5'- uACCAGaguUGGUGgcGAGcgUGGCGAUa- -3' miRNA: 3'- -UGGUC---AUCGCuaCUCuaACCGCUAcu -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 4125 | 0.7 | 0.901573 |
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Target: 5'- -aCGGUGGUGGUGGuGgcGGCGGUGAa -3' miRNA: 3'- ugGUCAUCGCUACUcUaaCCGCUACU- -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 122076 | 0.73 | 0.795105 |
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Target: 5'- --aGGUGGUGGUGGuGUUGGUGAUGAu -3' miRNA: 3'- uggUCAUCGCUACUcUAACCGCUACU- -5' |
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| 8515 | 3' | -50.8 | NC_002169.1 | + | 107900 | 1.09 | 0.007489 |
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Target: 5'- cACCAGUAGCGAUGAGAUUGGCGAUGAc -3' miRNA: 3'- -UGGUCAUCGCUACUCUAACCGCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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