Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8519 | 5' | -55.5 | NC_002169.1 | + | 44740 | 0.66 | 0.902854 |
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Target: 5'- aUAUGCCCUgCGugcgcagaaaGGCCGaaUGGGCGa -3' miRNA: 3'- -AUGCGGGAgGUuuaa------CCGGC--ACCUGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 121857 | 0.66 | 0.892299 |
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Target: 5'- -cCGCCaacggCCGAGcguuUUGGCCGgacgGGACa -3' miRNA: 3'- auGCGGga---GGUUU----AACCGGCa---CCUGc -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 87478 | 0.67 | 0.86177 |
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Target: 5'- -cCGCCCaucguUUCAAGUUGGCCauacacucgaucUGGACGg -3' miRNA: 3'- auGCGGG-----AGGUUUAACCGGc-----------ACCUGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 57084 | 0.67 | 0.855513 |
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Target: 5'- gUGCGCUguCUCuCGAGUUugacGGCgGUGGACa -3' miRNA: 3'- -AUGCGG--GAG-GUUUAA----CCGgCACCUGc -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 22642 | 0.67 | 0.847505 |
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Target: 5'- cUACGCCCgcuucCCAAGUgccgGGCaCG-GGGCc -3' miRNA: 3'- -AUGCGGGa----GGUUUAa---CCG-GCaCCUGc -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 56403 | 0.67 | 0.830891 |
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Target: 5'- gACGCCCg-CA--UUGGUCGUGGuaGCGa -3' miRNA: 3'- aUGCGGGagGUuuAACCGGCACC--UGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 22566 | 0.68 | 0.822299 |
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Target: 5'- gACGCCgCUCCAuccgcGGCCGUacauGGCGa -3' miRNA: 3'- aUGCGG-GAGGUuuaa-CCGGCAc---CUGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 96421 | 0.68 | 0.813529 |
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Target: 5'- uUGCGCaCCUUCuuGUUGuagagccggguGUCGUGGACGc -3' miRNA: 3'- -AUGCG-GGAGGuuUAAC-----------CGGCACCUGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 110410 | 0.7 | 0.707849 |
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Target: 5'- cACGCCCUU----UUGGCCGUGaACGc -3' miRNA: 3'- aUGCGGGAGguuuAACCGGCACcUGC- -5' |
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| 8519 | 5' | -55.5 | NC_002169.1 | + | 110378 | 1.07 | 0.003728 |
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Target: 5'- uUACGCCCUCCAAAUUGGCCGUGGACGu -3' miRNA: 3'- -AUGCGGGAGGUUUAACCGGCACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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