Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
852 | 3' | -60.6 | NC_000898.1 | + | 4342 | 0.66 | 0.754184 |
Target: 5'- gCCucauaGGUGGGGcauUCgaGGCCGCCCUCu -3' miRNA: 3'- -GGucug-CCGUCUC---AGa-CCGGCGGGAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 157509 | 0.66 | 0.754184 |
Target: 5'- gCCucauaGGUGGGGcauUCgaGGCCGCCCUCu -3' miRNA: 3'- -GGucug-CCGUCUC---AGa-CCGGCGGGAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 785 | 0.66 | 0.715433 |
Target: 5'- -aAGACGauGCAGGGUgUGGCCggcacgcGCCgUCa -3' miRNA: 3'- ggUCUGC--CGUCUCAgACCGG-------CGGgAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 153953 | 0.66 | 0.715433 |
Target: 5'- -aAGACGauGCAGGGUgUGGCCggcacgcGCCgUCa -3' miRNA: 3'- ggUCUGC--CGUCUCAgACCGG-------CGGgAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 123671 | 0.66 | 0.706758 |
Target: 5'- uCCcGACGGCAGAGguugcGGUUugGCCUUCg -3' miRNA: 3'- -GGuCUGCCGUCUCaga--CCGG--CGGGAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 62974 | 0.67 | 0.638105 |
Target: 5'- aUCGGACGGUgauGGGUUUGGCCauGCCg-- -3' miRNA: 3'- -GGUCUGCCGu--CUCAGACCGG--CGGgag -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 4497 | 0.69 | 0.559475 |
Target: 5'- gCC--GCGGCAGGGUCgGcGCCaGCCCaUCg -3' miRNA: 3'- -GGucUGCCGUCUCAGaC-CGG-CGGG-AG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 157665 | 0.69 | 0.559475 |
Target: 5'- gCC--GCGGCAGGGUCgGcGCCaGCCCaUCg -3' miRNA: 3'- -GGucUGCCGUCUCAGaC-CGG-CGGG-AG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 7195 | 0.72 | 0.404981 |
Target: 5'- gCCAGACGGCAGA-UCUuGCCGUuaUCg -3' miRNA: 3'- -GGUCUGCCGUCUcAGAcCGGCGggAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 160362 | 0.72 | 0.404981 |
Target: 5'- gCCAGACGGCAGA-UCUuGCCGUuaUCg -3' miRNA: 3'- -GGUCUGCCGUCUcAGAcCGGCGggAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 6658 | 1.11 | 0.000866 |
Target: 5'- gCCAGACGGCAGAGUCUGGCCGCCCUCa -3' miRNA: 3'- -GGUCUGCCGUCUCAGACCGGCGGGAG- -5' |
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852 | 3' | -60.6 | NC_000898.1 | + | 159826 | 1.11 | 0.000866 |
Target: 5'- gCCAGACGGCAGAGUCUGGCCGCCCUCa -3' miRNA: 3'- -GGUCUGCCGUCUCAGACCGGCGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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