Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 852 | 5' | -55.2 | NC_000898.1 | + | 25751 | 0.66 | 0.948783 |
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Target: 5'- uGCuUGCaACGGCUCCGUagacaaCUGCUGAg -3' miRNA: 3'- -UGcAUG-UGCCGAGGCAga----GAUGGCUg -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 5368 | 0.69 | 0.850753 |
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Target: 5'- cGCGgcCACGGUagguggaUCCGuUCUCcccggUGCCGACu -3' miRNA: 3'- -UGCauGUGCCG-------AGGC-AGAG-----AUGGCUG- -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 158535 | 0.69 | 0.850753 |
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Target: 5'- cGCGgcCACGGUagguggaUCCGuUCUCcccggUGCCGACu -3' miRNA: 3'- -UGCauGUGCCG-------AGGC-AGAG-----AUGGCUG- -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 1640 | 0.69 | 0.835458 |
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Target: 5'- uGCGUACACGGCcacCCGcgUCU-CCGAUc -3' miRNA: 3'- -UGCAUGUGCCGa--GGCagAGAuGGCUG- -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 154808 | 0.69 | 0.835458 |
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Target: 5'- uGCGUACACGGCcacCCGcgUCU-CCGAUc -3' miRNA: 3'- -UGCAUGUGCCGa--GGCagAGAuGGCUG- -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 6623 | 1.08 | 0.004284 |
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Target: 5'- gACGUACACGGCUCCGUCUCUACCGACc -3' miRNA: 3'- -UGCAUGUGCCGAGGCAGAGAUGGCUG- -5' |
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| 852 | 5' | -55.2 | NC_000898.1 | + | 159791 | 1.08 | 0.004284 |
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Target: 5'- gACGUACACGGCUCCGUCUCUACCGACc -3' miRNA: 3'- -UGCAUGUGCCGAGGCAGAGAUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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