Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8521 | 5' | -54.4 | NC_002169.1 | + | 9686 | 0.66 | 0.952475 |
Target: 5'- aUUGUUucccaCCGuuuCGUUGAgCGUCGUCGa -3' miRNA: 3'- gAACAA-----GGCuc-GCGACUgGCAGCAGC- -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 48378 | 0.66 | 0.945684 |
Target: 5'- -----aCCGAGUGCcggaucgcagucgaGAUCGUCGUCGg -3' miRNA: 3'- gaacaaGGCUCGCGa-------------CUGGCAGCAGC- -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 84939 | 0.67 | 0.90875 |
Target: 5'- -aUGUUUCGAGCGCgagagaGACacgugcuuucuaccgUGUCGUCa -3' miRNA: 3'- gaACAAGGCUCGCGa-----CUG---------------GCAGCAGc -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 37406 | 0.68 | 0.87739 |
Target: 5'- --cGUcggCCGAuCGCUguacacuccgaacaGACCGUCGUCGa -3' miRNA: 3'- gaaCAa--GGCUcGCGA--------------CUGGCAGCAGC- -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 127525 | 0.69 | 0.84924 |
Target: 5'- --cGUUUCGAGCGCcaUGAagcCCGUucaCGUCGa -3' miRNA: 3'- gaaCAAGGCUCGCG--ACU---GGCA---GCAGC- -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 133162 | 0.72 | 0.670787 |
Target: 5'- gUUGUUgCCGAGUauCUGcGCCGUCGUCGc -3' miRNA: 3'- gAACAA-GGCUCGc-GAC-UGGCAGCAGC- -5' |
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8521 | 5' | -54.4 | NC_002169.1 | + | 112751 | 1.09 | 0.004319 |
Target: 5'- aCUUGUUCCGAGCGCUGACCGUCGUCGc -3' miRNA: 3'- -GAACAAGGCUCGCGACUGGCAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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