Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8522 | 3' | -57.2 | NC_002169.1 | + | 108103 | 0.66 | 0.80027 |
Target: 5'- uUGCAGGAGCGaCGUUCUUAcuuuuguugCGCGGu -3' miRNA: 3'- uACGUUCUCGC-GCGGGAGUa--------GUGCCu -5' |
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8522 | 3' | -57.2 | NC_002169.1 | + | 45959 | 0.67 | 0.772371 |
Target: 5'- --uCGAGAGCGCGaCCgaCGUCuCGGAc -3' miRNA: 3'- uacGUUCUCGCGC-GGgaGUAGuGCCU- -5' |
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8522 | 3' | -57.2 | NC_002169.1 | + | 95323 | 0.67 | 0.753112 |
Target: 5'- uUGCGAGAuguugggaugGCGCGUCgUCcacgCGCGGAc -3' miRNA: 3'- uACGUUCU----------CGCGCGGgAGua--GUGCCU- -5' |
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8522 | 3' | -57.2 | NC_002169.1 | + | 114425 | 0.71 | 0.538553 |
Target: 5'- cGUGaUGAGGGCGCGCUCUUG-CAUGGAc -3' miRNA: 3'- -UAC-GUUCUCGCGCGGGAGUaGUGCCU- -5' |
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8522 | 3' | -57.2 | NC_002169.1 | + | 114584 | 1.06 | 0.002442 |
Target: 5'- cAUGCAAGAGCGCGCCCUCAUCACGGAc -3' miRNA: 3'- -UACGUUCUCGCGCGGGAGUAGUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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