Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8524 | 3' | -52.2 | NC_002169.1 | + | 53320 | 0.66 | 0.981326 |
Target: 5'- -cGACGGCGcCGCGGGuuacgaucuccACACCCccGuCGa -3' miRNA: 3'- guUUGCCGU-GCGCUU-----------UGUGGGuaC-GC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 32532 | 0.66 | 0.976673 |
Target: 5'- -cAACGGUACGCGccGCAgCC--GCGg -3' miRNA: 3'- guUUGCCGUGCGCuuUGUgGGuaCGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 51929 | 0.66 | 0.97642 |
Target: 5'- uGGACGGCACGCugauuGAGACGCgaaguggCCAaGUGu -3' miRNA: 3'- gUUUGCCGUGCG-----CUUUGUG-------GGUaCGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 96392 | 0.66 | 0.974051 |
Target: 5'- --cACGGCGCGCGAGugGCagaaUcguucgagagagGUGCGc -3' miRNA: 3'- guuUGCCGUGCGCUUugUGg---G------------UACGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 57779 | 0.66 | 0.970331 |
Target: 5'- ---uCGGCgucggacauuucgaACGCGAAGCGCgCcgGCGu -3' miRNA: 3'- guuuGCCG--------------UGCGCUUUGUGgGuaCGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 126316 | 0.67 | 0.968177 |
Target: 5'- aCGGGCGGC-CGCuuGACGCUCAacgaaGCGa -3' miRNA: 3'- -GUUUGCCGuGCGcuUUGUGGGUa----CGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 48189 | 0.67 | 0.967221 |
Target: 5'- -uAGCGGCAuCGCGGGcAgACCCGacgacgaucucgacUGCGa -3' miRNA: 3'- guUUGCCGU-GCGCUU-UgUGGGU--------------ACGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 115929 | 0.67 | 0.964911 |
Target: 5'- --cGCGGCAaGCGAGACGgCC--GCGa -3' miRNA: 3'- guuUGCCGUgCGCUUUGUgGGuaCGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 77767 | 0.67 | 0.961416 |
Target: 5'- --cGCGGCACGCGAuggugcAGCACCaa--CGa -3' miRNA: 3'- guuUGCCGUGCGCU------UUGUGGguacGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 21635 | 0.67 | 0.961416 |
Target: 5'- uGAGCGGCGCcagGUGGGAucCGCUCGUGuCGg -3' miRNA: 3'- gUUUGCCGUG---CGCUUU--GUGGGUAC-GC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 112182 | 0.68 | 0.940338 |
Target: 5'- aGAACGGCGCuuGCGuuuCGCCCGucauUGCc -3' miRNA: 3'- gUUUGCCGUG--CGCuuuGUGGGU----ACGc -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 1573 | 0.69 | 0.90674 |
Target: 5'- -cGGCGGCGCGUaaGAuuguuccGCACCCAUGg- -3' miRNA: 3'- guUUGCCGUGCG--CUu------UGUGGGUACgc -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 24533 | 0.69 | 0.900253 |
Target: 5'- -cGACGGCGCGaucgccaaCGuguACACCCAUGaCGc -3' miRNA: 3'- guUUGCCGUGC--------GCuu-UGUGGGUAC-GC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 80388 | 0.7 | 0.879321 |
Target: 5'- uGAGCGGCAuCGUGA-GCAUUgAUGCGc -3' miRNA: 3'- gUUUGCCGU-GCGCUuUGUGGgUACGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 80150 | 0.74 | 0.653742 |
Target: 5'- --cAUGuGCACGCGAGaAUugCCGUGCGa -3' miRNA: 3'- guuUGC-CGUGCGCUU-UGugGGUACGC- -5' |
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8524 | 3' | -52.2 | NC_002169.1 | + | 116176 | 1.09 | 0.006152 |
Target: 5'- aCAAACGGCACGCGAAACACCCAUGCGc -3' miRNA: 3'- -GUUUGCCGUGCGCUUUGUGGGUACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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