Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8524 | 5' | -51.9 | NC_002169.1 | + | 92774 | 0.66 | 0.983955 |
Target: 5'- -cCUCCUCGc-AAACgUCCCaUCACCa- -3' miRNA: 3'- caGAGGAGCuaUUUG-AGGG-AGUGGcg -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 133833 | 0.66 | 0.979793 |
Target: 5'- gGUCUCgaCGAUAAAUcuaaacaagUCUCUCGCCu- -3' miRNA: 3'- -CAGAGgaGCUAUUUG---------AGGGAGUGGcg -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 46061 | 0.67 | 0.974878 |
Target: 5'- aUCgUCUCGAU--GCUgUCCUCGCCGUc -3' miRNA: 3'- cAGaGGAGCUAuuUGA-GGGAGUGGCG- -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 106296 | 0.69 | 0.924265 |
Target: 5'- gGUUUCCUCaucaucaucGCUCCCgUACCGCa -3' miRNA: 3'- -CAGAGGAGcuauu----UGAGGGaGUGGCG- -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 134298 | 0.69 | 0.920852 |
Target: 5'- cGUCgaCCagggUGGUAAGCgaaaaggugCCCUCGCCGCc -3' miRNA: 3'- -CAGa-GGa---GCUAUUUGa--------GGGAGUGGCG- -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 22195 | 0.73 | 0.779708 |
Target: 5'- cGUCgCCcacgUCGGgguuGGCUCCCUUGCCGCc -3' miRNA: 3'- -CAGaGG----AGCUau--UUGAGGGAGUGGCG- -5' |
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8524 | 5' | -51.9 | NC_002169.1 | + | 116132 | 1.12 | 0.004442 |
Target: 5'- cGUCUCCUCGAUAAACUCCCUCACCGCc -3' miRNA: 3'- -CAGAGGAGCUAUUUGAGGGAGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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