Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8526 | 5' | -51.4 | NC_002169.1 | + | 24380 | 0.75 | 0.66122 |
Target: 5'- gUUGGCGAUCGCGCcGUCGaucaCGGGCGa -3' miRNA: 3'- aAACUGCUAGUGCGcCAGCa---GCUUGU- -5' |
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8526 | 5' | -51.4 | NC_002169.1 | + | 104205 | 0.76 | 0.629356 |
Target: 5'- cUUGugGG-CACGCucGGUCGUCGAAUg -3' miRNA: 3'- aAACugCUaGUGCG--CCAGCAGCUUGu -5' |
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8526 | 5' | -51.4 | NC_002169.1 | + | 123435 | 1.07 | 0.010267 |
Target: 5'- uUUUGACGAUCACGCGGUCGUCGAACAu -3' miRNA: 3'- -AAACUGCUAGUGCGCCAGCAGCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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