Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8529 | 5' | -56.2 | NC_002169.1 | + | 134370 | 0.67 | 0.866285 |
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Target: 5'- ---aGUCGGGCUCGcGUGCGUaaaGGUg -3' miRNA: 3'- guagCAGCUCGGGCaCAUGCAg--CCGu -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 128541 | 0.67 | 0.850934 |
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Target: 5'- --cCGUCGcGCCCGUGcuaaugaacUGCGaaggCGGCGa -3' miRNA: 3'- guaGCAGCuCGGGCAC---------AUGCa---GCCGU- -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 95219 | 0.69 | 0.724834 |
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Target: 5'- --cCGUCGcAGUCCGUGUuaGCGcCGGUg -3' miRNA: 3'- guaGCAGC-UCGGGCACA--UGCaGCCGu -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 9709 | 0.7 | 0.714938 |
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Target: 5'- gCGUCGUCGAacGUuuGUGUGagGUCGGUu -3' miRNA: 3'- -GUAGCAGCU--CGggCACAUg-CAGCCGu -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 107341 | 0.7 | 0.714938 |
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Target: 5'- --cCGUCagcggaGAGUCCGUGUACGacaacggCGGCAu -3' miRNA: 3'- guaGCAG------CUCGGGCACAUGCa------GCCGU- -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 86213 | 0.7 | 0.704972 |
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Target: 5'- -uUUGUCGAGCgCGUcGUAUGUCGcGUAc -3' miRNA: 3'- guAGCAGCUCGgGCA-CAUGCAGC-CGU- -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 125436 | 0.7 | 0.704972 |
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Target: 5'- aCGUCGUCGAuGCCCuugaugacaUGUuCGUCGGUg -3' miRNA: 3'- -GUAGCAGCU-CGGGc--------ACAuGCAGCCGu -5' |
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| 8529 | 5' | -56.2 | NC_002169.1 | + | 464 | 1.08 | 0.003293 |
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Target: 5'- gCAUCGUCGAGCCCGUGUACGUCGGCAc -3' miRNA: 3'- -GUAGCAGCUCGGGCACAUGCAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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