Results 101 - 107 of 107 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8530 | 5' | -60.2 | NC_002169.1 | + | 59663 | 0.66 | 0.689658 |
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Target: 5'- -aGUCGCCGUCGaaGCCaUCcUCGa- -3' miRNA: 3'- aaCAGCGGCAGCggCGGcAGcAGCag -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 84697 | 0.66 | 0.695573 |
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Target: 5'- aUGUCGCCaaacccucuacuuUCGCCGCCcG-CGUCGa- -3' miRNA: 3'- aACAGCGGc------------AGCGGCGG-CaGCAGCag -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 77908 | 0.66 | 0.699507 |
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Target: 5'- gUGcCGCgCGUCGCCGaCGaCG-CGUCg -3' miRNA: 3'- aACaGCG-GCAGCGGCgGCaGCaGCAG- -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 49431 | 0.66 | 0.699507 |
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Target: 5'- aUUGUaaaCGgCGUCGCCGUCGUUGaUGUUu -3' miRNA: 3'- -AACA---GCgGCAGCGGCGGCAGCaGCAG- -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 122159 | 0.66 | 0.699507 |
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Target: 5'- -cGUCGUCGUugaCGCUauuGCCGUCGcCGcCa -3' miRNA: 3'- aaCAGCGGCA---GCGG---CGGCAGCaGCaG- -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 62065 | 0.66 | 0.728697 |
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Target: 5'- -aGUCGaCGaaaagaCGCCGCCGUCGauUCGgUCa -3' miRNA: 3'- aaCAGCgGCa-----GCGGCGGCAGC--AGC-AG- -5' |
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| 8530 | 5' | -60.2 | NC_002169.1 | + | 75704 | 0.66 | 0.728697 |
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Target: 5'- --aUCGCCGUCaCCaUCGaCGUCGUCu -3' miRNA: 3'- aacAGCGGCAGcGGcGGCaGCAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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