Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8534 | 3' | -58.3 | NC_002169.1 | + | 109983 | 0.66 | 0.746822 |
Target: 5'- uGCAGguGCAGGGcGGGcaUGUGCUc -3' miRNA: 3'- uCGUCguCGUCCUcCUCcaACACGGa -5' |
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8534 | 3' | -58.3 | NC_002169.1 | + | 1447 | 0.67 | 0.706898 |
Target: 5'- cGguGguGgAGGAGGAGGUgGUGgUg -3' miRNA: 3'- uCguCguCgUCCUCCUCCAaCACgGa -5' |
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8534 | 3' | -58.3 | NC_002169.1 | + | 22241 | 0.68 | 0.614158 |
Target: 5'- cGCAGCucuuGCAGGAGGAGc--GUGUa- -3' miRNA: 3'- uCGUCGu---CGUCCUCCUCcaaCACGga -5' |
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8534 | 3' | -58.3 | NC_002169.1 | + | 7509 | 1.08 | 0.001417 |
Target: 5'- cAGCAGCAGCAGGAGGAGGUUGUGCCUg -3' miRNA: 3'- -UCGUCGUCGUCCUCCUCCAACACGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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