Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8541 | 3' | -50.7 | NC_002169.1 | + | 30480 | 0.75 | 0.710831 |
Target: 5'- ----cGGcaacGUGAgCGGCGGCGUCGUCGa -3' miRNA: 3'- guuaaCCa---CACU-GCUGUCGCAGCAGC- -5' |
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8541 | 3' | -50.7 | NC_002169.1 | + | 118949 | 0.77 | 0.605492 |
Target: 5'- ---aUGGuUGUuuaacGGCGGCGGCGUCGUCGu -3' miRNA: 3'- guuaACC-ACA-----CUGCUGUCGCAGCAGC- -5' |
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8541 | 3' | -50.7 | NC_002169.1 | + | 15806 | 1.09 | 0.008696 |
Target: 5'- aCAAUUGGUGUGACGACAGCGUCGUCGu -3' miRNA: 3'- -GUUAACCACACUGCUGUCGCAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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