Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8547 | 5' | -51.4 | NC_002169.1 | + | 73669 | 0.67 | 0.976702 |
Target: 5'- aUUguuCUCGCgUCAGCCAgaACAGCa- -3' miRNA: 3'- gAGau-GAGCG-AGUUGGUgaUGUCGcu -5' |
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8547 | 5' | -51.4 | NC_002169.1 | + | 1057 | 0.67 | 0.96482 |
Target: 5'- -gCUGCUCG-UCGACgACgguucgACGGCGAc -3' miRNA: 3'- gaGAUGAGCgAGUUGgUGa-----UGUCGCU- -5' |
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8547 | 5' | -51.4 | NC_002169.1 | + | 95062 | 0.69 | 0.91824 |
Target: 5'- -cCUACUCuuUCAACgACU-CAGCGAc -3' miRNA: 3'- gaGAUGAGcgAGUUGgUGAuGUCGCU- -5' |
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8547 | 5' | -51.4 | NC_002169.1 | + | 134607 | 0.69 | 0.91824 |
Target: 5'- gCUCaUGCgcgUCGCUCuGGCCAUUcACGGCGAc -3' miRNA: 3'- -GAG-AUG---AGCGAG-UUGGUGA-UGUCGCU- -5' |
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8547 | 5' | -51.4 | NC_002169.1 | + | 18284 | 0.7 | 0.912168 |
Target: 5'- -aUUGCUCGCgUCAugCACaGCGGCa- -3' miRNA: 3'- gaGAUGAGCG-AGUugGUGaUGUCGcu -5' |
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8547 | 5' | -51.4 | NC_002169.1 | + | 33638 | 1.09 | 0.0065 |
Target: 5'- gCUCUACUCGCUCAACCACUACAGCGAc -3' miRNA: 3'- -GAGAUGAGCGAGUUGGUGAUGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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