miRNA: 3'- -GCUGACGAgCCACAGcuuCACcUGCA- -5'
miRNA: 3'- -GCUGaCG-AGCCACag-------CUUCACCugCA- -5'
miRNA: 3'- -GCUGACGAGCCACAGCUUCACCUGCA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8549 | 5' | -54.5 | NC_002169.1 | + | 50367 | 0.69 | 0.819935 |
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Target: 5'- cCGACUGCUgGGUGUCc--GUGuACGUu -3' miRNA: 3'- -GCUGACGAgCCACAGcuuCACcUGCA- -5' |
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| 8549 | 5' | -54.5 | NC_002169.1 | + | 1437 | 0.7 | 0.765106 |
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Target: 5'- aCGACgGCaUCGGUGguggaggagGAGGUGGugGUg -3' miRNA: 3'- -GCUGaCG-AGCCACag-------CUUCACCugCA- -5' |
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| 8549 | 5' | -54.5 | NC_002169.1 | + | 35776 | 1.07 | 0.005408 |
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Target: 5'- uCGACUGCUCGGUGUCGAAGUGGACGUu -3' miRNA: 3'- -GCUGACGAGCCACAGCUUCACCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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