Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8550 | 5' | -55.4 | NC_002169.1 | + | 106377 | 0.66 | 0.901174 |
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Target: 5'- uGcgGUCuuugguaaauGUCUCGCGGCCAGucggugaaCGUCg -3' miRNA: 3'- -CuaCAGu---------CGGAGUGCCGGUCua------GCAG- -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 47975 | 0.66 | 0.894567 |
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Target: 5'- -cUGUCcguGUUUCAUGGCguGGUCGUa -3' miRNA: 3'- cuACAGu--CGGAGUGCCGguCUAGCAg -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 49879 | 0.66 | 0.894567 |
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Target: 5'- -uUGUCGGCCaugUCGCGGCaaa--CGUCg -3' miRNA: 3'- cuACAGUCGG---AGUGCCGgucuaGCAG- -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 118709 | 0.66 | 0.887024 |
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Target: 5'- aAUGUCAGCacaacaaUUCACGGCC-GAUCu-- -3' miRNA: 3'- cUACAGUCG-------GAGUGCCGGuCUAGcag -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 129958 | 0.67 | 0.833543 |
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Target: 5'- --gGUUuuuGGCgUCAaacuCGGCCAGAUCGUa -3' miRNA: 3'- cuaCAG---UCGgAGU----GCCGGUCUAGCAg -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 125495 | 0.68 | 0.779633 |
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Target: 5'- -cUGUC-GCCgcuuCGGCCGGAcucUCGUCa -3' miRNA: 3'- cuACAGuCGGagu-GCCGGUCU---AGCAG- -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 74127 | 0.69 | 0.730853 |
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Target: 5'- ---uUCGGCC-CACGGCC--GUCGUCu -3' miRNA: 3'- cuacAGUCGGaGUGCCGGucUAGCAG- -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 82712 | 0.74 | 0.466264 |
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Target: 5'- --cGUCAGagUCAacuuugacauguCGGCCAGAUCGUCg -3' miRNA: 3'- cuaCAGUCggAGU------------GCCGGUCUAGCAG- -5' |
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| 8550 | 5' | -55.4 | NC_002169.1 | + | 36877 | 1.1 | 0.002284 |
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Target: 5'- gGAUGUCAGCCUCACGGCCAGAUCGUCa -3' miRNA: 3'- -CUACAGUCGGAGUGCCGGUCUAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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