Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8554 | 3' | -55.8 | NC_002169.1 | + | 121991 | 0.72 | 0.596245 |
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Target: 5'- cGUGGCGGCGAcGGcaAUAGCGUCaacGACGa -3' miRNA: 3'- -CACUGCCGCU-CC--UGUCGUAGcu-CUGC- -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 6370 | 0.72 | 0.575846 |
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Target: 5'- -cGGCGGCGAcuACAGCGUCGAuGAUa -3' miRNA: 3'- caCUGCCGCUccUGUCGUAGCU-CUGc -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 12515 | 0.72 | 0.565703 |
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Target: 5'- gGUGGCGGCGuuGACGGU--CGAGGCu -3' miRNA: 3'- -CACUGCCGCucCUGUCGuaGCUCUGc -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 1332 | 0.73 | 0.555607 |
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Target: 5'- aUGACGGCGGcGGCGGCggCGGcGGCGg -3' miRNA: 3'- cACUGCCGCUcCUGUCGuaGCU-CUGC- -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 108724 | 0.76 | 0.387674 |
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Target: 5'- uGUGGCGGCGGcGGCGGCGaCGAcGACGa -3' miRNA: 3'- -CACUGCCGCUcCUGUCGUaGCU-CUGC- -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 57102 | 0.8 | 0.227371 |
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Target: 5'- uUGACGGCGGuGGACAGU-UCGAGGCu -3' miRNA: 3'- cACUGCCGCU-CCUGUCGuAGCUCUGc -5' |
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| 8554 | 3' | -55.8 | NC_002169.1 | + | 45899 | 1 | 0.011538 |
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Target: 5'- gGUGACGGCG-GGACAGCAUCGAGACGa -3' miRNA: 3'- -CACUGCCGCuCCUGUCGUAGCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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