Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8558 | 3' | -49.7 | NC_002169.1 | + | 107769 | 0.66 | 0.996023 |
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Target: 5'- cGUCGUcuccccgcUGCgGUGCGCuGUUUGGcCGUGAa -3' miRNA: 3'- -UAGCA--------AUG-CAUGCG-CAAGCC-GCAUUa -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 43064 | 0.66 | 0.994545 |
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Target: 5'- gAUUGUUgACGgugAUGCGUuugucgUCGGCGUAc- -3' miRNA: 3'- -UAGCAA-UGCa--UGCGCA------AGCCGCAUua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 16396 | 0.66 | 0.994545 |
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Target: 5'- uGUUGUUuuucgGCGUcaACGC-UUCGGCGUAu- -3' miRNA: 3'- -UAGCAA-----UGCA--UGCGcAAGCCGCAUua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 78148 | 0.67 | 0.992652 |
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Target: 5'- gAUCG--GCGUugGUccGUUUGGCGUAc- -3' miRNA: 3'- -UAGCaaUGCAugCG--CAAGCCGCAUua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 14863 | 0.67 | 0.990135 |
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Target: 5'- -aUGUUACGaGCacgacuaGCGUUgCGGCGUAAUa -3' miRNA: 3'- uaGCAAUGCaUG-------CGCAA-GCCGCAUUA- -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 67060 | 0.68 | 0.98372 |
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Target: 5'- -aCGUUACuGUcCGCGUagUCGGUGUGu- -3' miRNA: 3'- uaGCAAUG-CAuGCGCA--AGCCGCAUua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 58225 | 0.68 | 0.979398 |
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Target: 5'- -gCGaaACGUGCGUGUUCaGCGUAc- -3' miRNA: 3'- uaGCaaUGCAUGCGCAAGcCGCAUua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 40168 | 0.69 | 0.968289 |
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Target: 5'- -aCGUUcACGUACGCGg-CGGCGa--- -3' miRNA: 3'- uaGCAA-UGCAUGCGCaaGCCGCauua -5' |
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| 8558 | 3' | -49.7 | NC_002169.1 | + | 59563 | 1.05 | 0.017605 |
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Target: 5'- gAUCGUUACGUACGCGUUCGGCGUAAUa -3' miRNA: 3'- -UAGCAAUGCAUGCGCAAGCCGCAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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