Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
856 | 5' | -56.8 | NC_000898.1 | + | 9260 | 0.66 | 0.88695 |
Target: 5'- -cGAGCG-CACCgcggCCgCgGUGGCGUAg -3' miRNA: 3'- gaCUUGCuGUGGa---GG-GaCACCGCGUa -5' |
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856 | 5' | -56.8 | NC_000898.1 | + | 86995 | 0.67 | 0.825277 |
Target: 5'- -gGAACGACAUgUCUCUGcGGCGa-- -3' miRNA: 3'- gaCUUGCUGUGgAGGGACaCCGCgua -5' |
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856 | 5' | -56.8 | NC_000898.1 | + | 8915 | 0.7 | 0.642186 |
Target: 5'- gUGAaaaacACGGCGCCUCCCauagaGGCGCGc -3' miRNA: 3'- gACU-----UGCUGUGGAGGGaca--CCGCGUa -5' |
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856 | 5' | -56.8 | NC_000898.1 | + | 162083 | 0.7 | 0.642186 |
Target: 5'- gUGAaaaacACGGCGCCUCCCauagaGGCGCGc -3' miRNA: 3'- gACU-----UGCUGUGGAGGGaca--CCGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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