Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8564 | 3' | -60.3 | NC_002169.1 | + | 112210 | 0.66 | 0.703976 |
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Target: 5'- gGCCGCgU-CCAUC-CGGCgcUGCUCUa -3' miRNA: 3'- -CGGCGgGcGGUAGaGCCGauGCGAGA- -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 26960 | 0.66 | 0.684234 |
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Target: 5'- cGCCGCCCGa-AUCUUGGCcgucuucccgACGCcCg -3' miRNA: 3'- -CGGCGGGCggUAGAGCCGa---------UGCGaGa -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 46130 | 0.67 | 0.643318 |
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Target: 5'- -aCGCUCGCCcaGUCcgagacgUCGGUcGCGCUCUc -3' miRNA: 3'- cgGCGGGCGG--UAG-------AGCCGaUGCGAGA- -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 30834 | 0.67 | 0.614259 |
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Target: 5'- cGCCGCCCGUCAagUCaCGGgccguCUGCGUUUc -3' miRNA: 3'- -CGGCGGGCGGU--AGaGCC-----GAUGCGAGa -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 53459 | 0.68 | 0.584312 |
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Target: 5'- cGUCGCUgGCCAUUcgCGGCguauacGCGCUUUu -3' miRNA: 3'- -CGGCGGgCGGUAGa-GCCGa-----UGCGAGA- -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 60625 | 0.68 | 0.564502 |
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Target: 5'- cGCCGgacauugCCGCCAUCUCGGCgcugUGCa-- -3' miRNA: 3'- -CGGCg------GGCGGUAGAGCCGau--GCGaga -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 78279 | 0.69 | 0.506334 |
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Target: 5'- cGCUGCCCGCUGUCaUUcGCUACGUg-- -3' miRNA: 3'- -CGGCGGGCGGUAG-AGcCGAUGCGaga -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 49705 | 0.7 | 0.450879 |
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Target: 5'- uGCC-CCCGCCAUUggCGGCgACGC-Cg -3' miRNA: 3'- -CGGcGGGCGGUAGa-GCCGaUGCGaGa -5' |
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| 8564 | 3' | -60.3 | NC_002169.1 | + | 79815 | 1.08 | 0.001268 |
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Target: 5'- gGCCGCCCGCCAUCUCGGCUACGCUCg -3' miRNA: 3'- -CGGCGGGCGGUAGAGCCGAUGCGAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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